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KMID : 0903619830240020149
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Journal of the Korean Society for Horticultural Science
1983 Volume.24 No. 2 p.149 ~ p.157
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Factors Affecting Regeneration Ability and Physiology of Dormancy in the Mature Bulbil Segments of Lilium Iancifolium in vitro
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Abstract
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This in vitro study was performed to clarify the physiology of dormzncy and factors affecting the bulblets regeneration of Lilium lancifolium bulbil; i.e., 1. Difference of regeneration ability as related to the origin of explant from bulbil. 2, Influence of gibberellic acid(GA₃), abscisic acid(ABA), and 4℃ chilling treatment(CT) on the bulblet regeneration and dormacy breaking in bulbil-scale explant. 3. The effect of chilling treatment on the scale leaf emergence for subsequent growth of produced bulblet in vitro
1. Bulb regeneration from hulbil-scale explant was originated in the epidermis and/or subepidermis cells of adaxial side of scale.
2. Bulb and root formation and subsequent growth of the generated bulb was greatly promoted in the distal part of outer scale segment compared to the bzsal or distal part of inner scale segmennt compared to the basal or dital part of inner scale segment. This indicated that there is some gradient in regeneration power and endogenous growth substance by the origin of explant in the mature bulbil.
3. Scale segment which induced dormancy by adding ABA into the medium inhibited both organegenesis and bulblet weight whereas GA₃ markedly increased both organogenesis and growth of bulblet; 1.0㎎/ℓ GA₃ acted much stronger than the other levels of GA₃, ABA and control
4. Exposure of bulbil-scale explant at constant 4℃ for 30 days after pretreatment of bulbil with 100ppm GA₃ promoted bulblet regeneration and bulb weight as in control but pretreatment of the of bulbil with 200ppm GA₃ was effective in breaking dormancy of the bulbil and produced the largest number of bulblets and bulb weight per explant at chilling treatment for 10days. Pretreatment of bulbil with GA₃ before tissue culture supposed to be stimulation of bulblet regeneration and growth compared to control.
5. In order to transfer in vitro produced bulbs to in vivo, chilling treatment should be given for 30days or longer duration to break their dormancy and to cause emergence of scale leaf which is necessary for continuous bathing of bulblet formed.
6. This data suggested that physiological states and dormancy of bulbil-scale segments during in vitro development were critical factors for bulb regeneration, and temperature and its duration affected scale leaf emergence as measured by leaf production.
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